anti trim21 (Proteintech)
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Proteintech
anti trim21
Anti Trim21, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+trim21/TRIM21+Antibody/pmc13047273-317-12-13
Average 96 stars, based on 152 article reviews
Anti Trim21, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+trim21/TRIM21+Antibody/pmc13047273-317-12-13
Average 96 stars, based on 152 article reviews
anti trim21 - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma. Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 ° C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation. Article Snippet: Proteins (10–30 μg/well, equal amounts within group but different amounts among groups for better comparison) were separated on 10% or 12% SDS-PAGE gel and transferred on an equilibrated polyvinylidene difluoride membrane (PVDF, Amersham Biosciences, Buckinghamshire, 8 www.thelancet.com Vol 125 March, 2026 UK). .. After blocking with 5% bovine serum albumin (BSA, Sigma–Aldrich) in TBST (10 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, the membrane was incubated with primary antibody overnight at 4 ◦ C. The primary antibodies contained anti-MFF (17090-1-AP, proteintech, dilution 1:1000, RRID: AB_2142463), Article Title: FAM135B Deficiency Inhibits Cytotoxic T-cell Activity in Triple-Negative Breast Cancer by Blocking the IFI16-Dependent STING Pathway Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore). .. The membranes were blocked in PBS buffer supplemented with 0.1% Tween containing 5% nonfat milk for 1 hour at room temperature, followed by overnight incubation with the specified primary antibodies at 4°C: anti-FAM135B (28061-1-AP, Proteintech), anti-IFI16 (YT2274, Immunoway), Control:Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma. Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 ° C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), Immunofluorescence:Article Title: E3 ubiquitin ligase TRIM21-mediated K48-linked ubiquitination of ALDH2 rs671 mutant promotes adverse cardiac remodeling. Article Snippet: Signals were visualized with Alexa427 fluorescence-conjugated secondary antibodies (Invitrogen, Carlsbad, CA,428 USA). .. The primary antibodies used for cell immunofluorescence are429 listed below: anti-CD68 (ab213363, Abcam, 1:200), anti-Rac2430 (60077-1-Ig, ProteinTech, 1:200), RNA Extraction:Article Title: E3 ubiquitin ligase TRIM21-mediated K48-linked ubiquitination of ALDH2 rs671 mutant promotes adverse cardiac remodeling. Article Snippet: Signals were visualized with Alexa427 fluorescence-conjugated secondary antibodies (Invitrogen, Carlsbad, CA,428 USA). .. The primary antibodies used for cell immunofluorescence are429 listed below: anti-CD68 (ab213363, Abcam, 1:200), anti-Rac2430 (60077-1-Ig, ProteinTech, 1:200), Real-time Polymerase Chain Reaction:Article Title: E3 ubiquitin ligase TRIM21-mediated K48-linked ubiquitination of ALDH2 rs671 mutant promotes adverse cardiac remodeling. Article Snippet: Signals were visualized with Alexa427 fluorescence-conjugated secondary antibodies (Invitrogen, Carlsbad, CA,428 USA). .. The primary antibodies used for cell immunofluorescence are429 listed below: anti-CD68 (ab213363, Abcam, 1:200), anti-Rac2430 (60077-1-Ig, ProteinTech, 1:200), Polymerase Chain Reaction:Article Title: E3 ubiquitin ligase TRIM21-mediated K48-linked ubiquitination of ALDH2 rs671 mutant promotes adverse cardiac remodeling. Article Snippet: Signals were visualized with Alexa427 fluorescence-conjugated secondary antibodies (Invitrogen, Carlsbad, CA,428 USA). .. The primary antibodies used for cell immunofluorescence are429 listed below: anti-CD68 (ab213363, Abcam, 1:200), anti-Rac2430 (60077-1-Ig, ProteinTech, 1:200), Western Blot:Article Title: E3 ubiquitin ligase TRIM21-mediated K48-linked ubiquitination of ALDH2 rs671 mutant promotes adverse cardiac remodeling. Article Snippet: Finally, protein blots were detected using an455 enhanced chemiluminescence (ECL) kit (Merck Millipore, USA) and456 imaging system (Thermo Fisher Scientific, USA), and the data were457 analyzed using Image J software. .. The primary antibodies used for458 western blotting are listed below: anti-ALDH2 (15310-1-AP, ProteinTech,459 1:1000), Blocking Assay:Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation. Article Snippet: Proteins (10–30 μg/well, equal amounts within group but different amounts among groups for better comparison) were separated on 10% or 12% SDS-PAGE gel and transferred on an equilibrated polyvinylidene difluoride membrane (PVDF, Amersham Biosciences, Buckinghamshire, 8 www.thelancet.com Vol 125 March, 2026 UK). .. After blocking with 5% bovine serum albumin (BSA, Sigma–Aldrich) in TBST (10 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, the membrane was incubated with primary antibody overnight at 4 ◦ C. The primary antibodies contained anti-MFF (17090-1-AP, proteintech, dilution 1:1000, RRID: AB_2142463), Membrane:Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation. Article Snippet: Proteins (10–30 μg/well, equal amounts within group but different amounts among groups for better comparison) were separated on 10% or 12% SDS-PAGE gel and transferred on an equilibrated polyvinylidene difluoride membrane (PVDF, Amersham Biosciences, Buckinghamshire, 8 www.thelancet.com Vol 125 March, 2026 UK). .. After blocking with 5% bovine serum albumin (BSA, Sigma–Aldrich) in TBST (10 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, the membrane was incubated with primary antibody overnight at 4 ◦ C. The primary antibodies contained anti-MFF (17090-1-AP, proteintech, dilution 1:1000, RRID: AB_2142463), |
